Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ANGPT2 in samples. An antibody specific for ANGPT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANGPT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Angiopoietin-2 (Ang-2) is a secreted glycoprotein that plays a complex role in vascular development. Ang-2 contains an N-terminal coiled-coildomain, 58 - 254 amino acids (aa) and a C-terminal fibrinogen-like domain, 281 - 467 aa, that is also found in other proteins including fibrinogen, tenascin, and ficolin. Located at the N-terminus are stretches of hydrophobic residues typical of secretory signal sequences. While Ang-2 is widely expressed in the mouse embryo, it is restricted postnatally to pro-angiogenic regions including the placenta, ovaries, and uterus. Ang-2 has been implicated in cancer development due to its role in angiogenesis.Ang-2-mediated destabilization of mature vessel structures accompanied by the presence of VEGF, may contribute to the angiogenesis associated with tumor tissues.
