Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AHSG in samples. An antibody specific for AHSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAHSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AHSG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHSG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-2-HS-glycoprotein is a protein which is encoded by the AHSG gene.Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.
