Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate B2-GP in samples. An antibody specific for B2-GP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB2-GP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B2-GP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B2-GP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Apolipoprotein H (ApoH), also known as Beta 2-glycoprotein I, is a plasma glycoprotein either circulating as a free protein or associated to lipoproteins. This protein was found in human serum and described for the first time in 1961 by Schultze et al. ApoH is a 54-kDa single-chain glycoprotein consisting of five carbohydrate chains and 326 amino acid residues that can be divided into five short consensus repeat domains.ApoH has been chiefly focused on as the cofactor or autoantigen in antiphospholipid syndrome (APS), which is related to venous and arterial thrombosis, fetal loss, and thrombocytopenia. Reports show that ApoH may have an important function in blood coagulation and clearance of apoptotic bodies from the circulation.
