Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate B-OHB in samples. An antibody specific for B-OHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-OHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-OHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-OHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Beta-Hydroxybutyric acid is a ketone body. It is a chiral compound having two enantiomers,?D-3-hydroxybutyric acid and?L-3-hydroxybutyric acid. Like the other ketone bodies, levels of?beta-hydroxybutyrate are raised in ketosis. In humans,?beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA, and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis. In alcoholic ketoacidosis, this ketone body is produced in greatest concentration. Both types of ketoacidosis result in an increase B-Hydroxybutyrate to oxaloacetate ratio, resulting in TCA cycle stalling and shifting of glucose towards ketone body production.Industrially, it can also be used for the synthesis of biodegradable plastics, such as poly. This polymer can be produced biologically by the bacteria, Alcaligenes eutrophus.
