Detection Method:Competitive ELISA
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASP. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated ASP and incubated. The competitive inhibition reaction is launched between with HRP labeled ASP and unlabeled ASP with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ASP in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview:Acylation Stimulating Protein (ASP) is one of the factors that control adipose tissue function. It was initially identified from human plasma, and determined to be a derivative of the 3rd complement component (C3). Arginine removal from activated C3 (C3a) by carboxypeptidase results in C3a-desArg, also known as ASP.Human fat cells have been shown to have mRNA for Adipsin (complement factor D), C3 and factor B, all of which are components necessary to generate C3a, the precursor for ASP. Basal production of ASP in adipocytes is dependent on adipocyte differentiation, with ASP production increasing after 7 days of adipocyte differentiation. While ASP is produced by the interaction of C3, factor B and Adipsin in the alternate complement pathway, it can also be potentially generated by the classical complement pathway as well as the lectin pathway.
