Detection Method:Competitive ELISA
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to aPT1/aPT2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated aPT1/aPT2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled aPT1/aPT2-Ab and unlabeled aPT1/aPT2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of aPT1/aPT2-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview:Fas is a glycoprotein with a mass estimated at 43 to 48 kDa . Fas is a member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF), and it shares a cytoplasmic motif with TNF RI, referred to as the death domain, that binds cytoplasm signaling molecules to trigger the cytoplasmic apoptotic signal . Fas is expressed to a large extent on activated T and B lymphocytes, and on malignant lymphoid cells. To a lesser extent, Fas is expressed on cells from liver, heart, kidney, ovaries, and on many other malignant cells. Yan et al. (2005) noted that the TNFRSF6 gene contains 9 exons. They identified an antisense transcript SAF within the 12.1-kb intron 1 that is transcribed in the opposite direction as the TNFRSF6 gene.
