Detection Method:Competitive ELISA
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-IL6 autoantibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-IL6 autoantibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Anti-IL6 autoantibody and unlabeled Anti-IL6 autoantibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Anti-IL6 autoantibody in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview:IL-6 is a multifunctional protein produced by lymphoid and non-lymphoid cells, and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these various cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. On the basis of its various activities, IL-6 has also been called interferon-β2 (IFN-β2), 26 kDa protein, B-cell stimulatory factor-2 (BSF-2),hybridoma/plasmacytoma growth factor, hepatocyte stimulating factor, cytotoxic T-cell differentiation factor, and macrophage-granulocyte inducing factor 2A (MGI-2A).
