Detection Method:Competitive ELISA
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EMAb. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated EMAb and incubated. The competitive inhibition reaction is launched between with HRP labeled EMAb and unlabeled EMAb with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of EMAb in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview:Anti-endometrial antibodies have been detected in the serum of women with endometriosis using immunofluorescence (Mathur et al, 1982; Wild and Shivers, 1985), immunodiffusion (Badawy et al, 1984), passive haemagglutination (Chihal et al, 1986; Badawy et al, 1990), enzyme-linked immunosorbent assay (ELISA) (Kennedy et al, 1990b) and Western blots (Mathur et al, 1988, 1990). Most studies show a higher prevalence of autoantibodies in women with endometriosis than in controls.However, Switchenko et al (1991), failed to demonstrate consistently higher concentrations of antiendometrial antibodies in women with endometriosis using immunofluorescence, haemagglutination, ELISA, or Western blotting, and concluded mat the case for specific anti-endometrial antibodies remains unproven.
