Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MPZ-Ab-IgG in samples. An antibody specific for MPZ-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPZ-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPZ-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPZ-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Myelin protein-zero is the major structural protein of peripheral myelin.Hayasaka et al. (1993) stated that the MPZ gene contains 6 exons.You et al. (1991) found that the Mpz gene in the mouse, like that in the rat, contains 6 exons that span about 7 kb of genomic DNA.Myelin protein-zero is the major structural protein of peripheral myelin, accounting for more than 50% of the protein present in the sheath of peripheral nerves. Expression of the MPZ gene is restricted to Schwann cells; MPZ is not found in the CNS. An integral membrane glycoprotein of 28 kD, MPZ is thought to link adjacent lamellae and thereby stabilize the myelin assembly. The other 3 major components of myelin are myelin basic protein (MBP), myelin proteolipid protein (PLP1), and myelin-associated glycoprotein (MAG).
