Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CCL11 in samples. An antibody specific for CCL11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:An eosinophil chemotactic factor (ECF) can be released from human polymorphonuclear neutrophils (PMN), rat mononuclear and rat mast cells by the calcium ionophore, during phagocytosis, by arachidonic acid and phospholipase A2. It has been suggested that stimuli such as the ionophore and the phagocytic event lead to phospholipid turnover with the generation of arachidonic acid which is subsequently transformed by a lipoxygenase-like enzyme into ECF. Chemokine (C-C motif) ligand 11 (CCL11) is a small cytokine belonging to the CC chemokine family that is also known as eotaxin-1. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses.
