• Product NameLysosomal acid lipase/cholesteryl ester hydrolase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Lysosomal acid lipase/cholesteryl ester hydrolase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Lysosomal acid lipase/cholesteryl ester hydrolase protein
• Target NameLysosomal acid lipase/cholesteryl ester hydrolase
• Alternative Names    Cholesteryl esterase Lipase A Sterol esterase LIPA
• Accession No. Swiss-Prot#: P38571
• UniprotP38571
• Gene ID3988;
• Calculated MW 42kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.

[1]Cloning and expression of cDNA encoding human lysosomal acid lipase/cholesteryl ester hydrolase. Similarities to gastric and lingual lipases.Anderson R.A., Sando G.N.J. Biol. Chem. 266:22479-22484(1991) [2]Purification, characterization and molecular

• Western blot All lanes:Lysosomal acid lipase/cholesteryl ester hydrolase antibody at 2ug/ml Lane 1:MCF-7 whole cell lysate Lane 2:K562 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :42kDa observed band size :42kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting