Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ARG in samples. An antibody specific for ARG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyARG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Arginine is an α-amino acid. The?L-form is one of the 20 most common natural amino acids. Arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. Infants are unable to meet their requirements and thus arginine is nutritionally essential for infants.Arginine was first isolated from a lupin seedling extract in 1886 by the Swiss chemist Ernst Schultze.The amino acid side chain of arginine consists of a 3-carbon aliphatic straight chain, the distal end of which is capped by a complex guanidinium group.With a pKa?of 12.48, the guanidinium group is positively charged in neutral, acidic and even most basic environments, and thus imparts basic chemical properties to arginine. Because of the conjugation between the double bond and the nitrogen lone pairs, the positive charge is de-localized, enabling the formation of multiple H-bonds.
