Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BCL2 in samples. An antibody specific for BCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Bcl-2) was one of the first oncogenic proteins discovered to inhibit apoptosis. The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein associated with cell survival.Bcl-2 is localized within the outer mitochondrial membrane, endoplasmic reticulum, and the nuclear envelope, where it exerts cell survival functions within many cell types. These functions include ion-conducting channel formation, integral protection from cytotoxic agents, inhibition of proteins that activate caspases responsible for disassembling cells, as well as the ability to modulate cell cycle initiation.
