Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BMP6 in samples. An antibody specific for BMP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Bone morphogenetic proteins, such as BMP6, belong to the transforming growth factor-beta superfamily of regulatory molecules (Rickard et al., 1998).Rickard et al. (1998) presented evidence that the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP6 by osteoblasts. They investigated the effect of estrogen on BMP production in 2 estrogen-responsive, human immortalized cell lines that display the mature osteoblast phenotype.Tamada et al. (1998) found that the promoter region of the BMP6 gene lacks a canonical TATA box, but has a GC-rich region with 2 SP1-binding sites, an inverted CCAAT element, and a putative tramtrack responsive element. Reporter gene assays suggested that the BMP6 promoter has osteogenic cell specificity.
