Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HIS in samples. An antibody specific for HIS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Histamine is the most important mediator and is mostly found in the initial phase of an anaphylactic reaction ("immediate type" allergy). Histamine is developed by the enzymatic decarboxylation of histidine. In the organism, histamine is present in nearly all tissues, and it is mainly stored in the metachromatic granula of mast cells and the basophilic leukocytes.It is present in an inactive bound form and is only released as required. Histamine acts predominantly on smooth muscle and blood vessels.It is responsible for the bronchoconstriction occurring during the acute phase. In the vessels, its constrictive effect is limited to the venula, whereas arterioles are dilated. Furthermore, histamine causes a contraction of the cells of the vascular endothelium and increases the vascular permeability, thereby allowing higher-molecular substances to escape into the tissue.
