Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GAL in samples. An antibody specific for GAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Galanin is formed by the cleavage of a prepropeptide encoded by a gene known as GAL. It is involved in a number of physiological processes such as regulation of food intake, metabolism and reproduction, regulation of neurotransmitter and hormone release, nociception, intestinal contraction and secretion, and more recently in nervous system development and response to injury. This wide diversity of action is mediated by several galanin receptor subtypes.Galanin is predominantly an inhibitory, hyperpolarizing neuropeptide and as such inhibits neurotransmitter release. Galanin is often co-localized with classical neurotransmitters such as acetylcholine, serotonin and norepinephrine and also with other neuromodulators such as Neuropeptide Y, Substance P and Vasoactive intestinal peptide.
