Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NFKBIE in samples. An antibody specific for NFKBIE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKBIE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKBIE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKBIE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins.NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex.For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).
