Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate XIAP in samples. An antibody specific for XIAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXIAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XIAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:X-linked Inhibitor of Apoptosis Protein (XIAP) is a member of the Inhibitor of apoptosis family of proteins (IAP). IAPs were initially identified in baculoviruses, but XIAP is one of the homologous proteins found in mammals. It is so called because it was first discovered by a 273 base pair site on the X chromosome.The protein is also called human IAP-like Protein (hILP), because it is not as well conserved as the human IAPS: hIAP-1 and hIAP-2. XIAP is the most potent human IAP protein currently identified.XIAP, like the rest of the IAP family, has two major structural elements. Firstly, there is the baculoviral IAP repeat (BIR) domain consisting of approximately 70 amino acids. Secondly, there is a zinc-binding domain, or a carboxy-terminal RING Finger.
