Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UBE2D2 in samples. An antibody specific for UBE2D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2D2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2D2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.Ubiquitin-conjugating enzyme E2 D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. UBE2D2 has been shown to interact with PJA1, Baculoviral IAP repeat-containing protein 3, PJA2, UBE3A and NEDD4.
