Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SLPI in samples. An antibody specific for SLPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Antileukoproteinase is secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.Secretory leukocyte protease inhibitor (SLPI) is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase.
