• Product NameRho guanine nucleotide exchange factor 7 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Rho guanine nucleotide exchange factor 7 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Human Rho guanine nucleotide exchange factor 7 protein
• Target NameRho guanine nucleotide exchange factor 7
• Alternative Names    Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB ORF Names: Nbla10314
• Accession No. Swiss-Prot#: Q14155
• UniprotQ14155
• Gene ID8874;
• Calculated MW 90kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions By similarity. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.

[1] Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1.Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N.DNA Res. 2:1

• All lanes : Rho guanine nucleotide exchange factor 7 antibody at 2ug/ml Lane 1 : mouse skeletal muscle tissue Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size:90KDa Observed band size:90KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting