Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ADAM19 in samples. An antibody specific for ADAM19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ADAM19?encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing results in two transcript variants.
