Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GPI in samples. An antibody specific for GPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Glucose-6-phosphate isomerase has an essential function in both catabolic glycolysis and anabolic gluconeogenesis and is universally distributed among Eukaryotes, Bacteria, and some Archaea.GPI is expressed by the corpus luteum on days 6C9 of pregnancy, the time at which implantation-promoting activity has been found in corpora lutea. Passive immunization against GPI reduced the number of implantation sites in pregnant ferrets in a dose-dependent manner. GPI is a multifunctional protein. Although first identified for its role in glycolysis, GPI has since been implicated in neural growth, lymphocyte maturation, and metastasis. This study demonstrates a previously uncharacterized function of this protein that may represent the natural motility-stimulating activity that has been co-opted by tumor cells.
