Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HDAC2 in samples. An antibody specific for HDAC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHDAC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HDAC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDAC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Histone deacetylase 2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events.YY1 is a mammalian zinc finger transcription factor that acts as both a positive and negative regulator of transcription at CCATNTT consensus DNA elements in promoters of cellular and viral genes.
