Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BAX in samples. An antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo.Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade.
