Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HMOX1 in samples. An antibody specific for HMOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHMOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; free iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5- or 3- UTRs of the mRNAs.
