Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
[1] "The primary structure of the 32-kDa subunit of human replication protein A."Erdile L.F., Wold M.S., Kelly T.J.J. Biol. Chem. 265:3177-3182(1990) [2] "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L
