Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CXCL9 in samples. An antibody specific for CXCL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCXCL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs, such as PDE8B. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families.Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates.
