Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VMA in samples. An antibody specific for VMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Moderately elevated VMA (vanillylmandelic acid) can be caused by a variety of factors such as essential hypertension, intense anxiety, intense physical exercise, and numerous drug interactions (including some over-the-counter medications and herbal products). Medications that may interfere with catecholamines and their metabolites include amphetamines and amphetamine-like compounds, appetite suppressants, bromocriptine, buspirone, caffeine, chlorpromazine, clonidine, disulfiram, diuretics (in doses sufficient to deplete sodium), epinephrine, glucagon, guanethidine, histamine, hydrazine derivatives, imipramine, levodopa , lithium, MAO inhibitors, melatonin, methyldopa , morphine, nitroglycerin, nose drops, propafenone, radiographic agents, rauwolfia alkaloids (Reserpine), tricyclic antidepressants, and vasodilators. The effects of some drugs on catecholamine metabolite results may not be predictable.
