• Product NameV-type proton ATPase subunit E 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total V-type proton ATPase subunit E 1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human V-type proton ATPase subunit E 1 protein
• Target NameV-type proton ATPase subunit E 1
• Alternative Names    ATP6V1E1
• Accession No. Swiss-Prot#: P36543
• UniprotP36543
• Gene ID529;
• Calculated MW 25kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

[1] "Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney."Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S.J. Biol. Chem. 267:9948-9957(1992) [2] "The

• V-type proton ATPase subunit E 1 Antibody at 2ug/ml + 293T whole cell lysate at 20ug SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 25 kDa Observed band size : 36 kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting