• Product NamePRAK antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 197 and 467 of PRAK
• Target NamePRAK
• Accession No. Swiss-Prot:Q8IW41
  Gene ID:8550
• UniprotQ8IW41
• Gene ID8550;
• Concentration 0.6mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 54kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: 293T
  B: H1299
  7.5% SDS PAGE
  Primary antibody diluted at 1: 500
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRAK antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting