• Product NameD-3-phosphoglycerate dehydrogenase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total D-3-phosphoglycerate dehydrogenase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human D-3-phosphoglycerate dehydrogenase protein
• Target NameD-3-phosphoglycerate dehydrogenase
• Alternative Names    3-PGDH??PGDH3??PHGDH
• Accession No. Swiss-Prot#: O43175
• UniprotO43175
• Gene ID26227;
• Calculated MW 59kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

PHGDH or 3-phosphoglycerate dehydrogenase is an enzyme belonging to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalysis the reaction 3-phosphoglycerate + NAD+ = 3-phosphonooxypyruvate + NADH during L-serine biosynthesis. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation and seizures.

[1] "Nucleotide sequence and differential expression of the human 3-phosphoglycerate dehydrogenase gene."Cho H.M., Jun D.Y., Bae M.A., Ahn J.D., Kim Y.H.Gene 245:193-201(2000) [2] "Molecular characterization of 3-phosphoglycerate dehyd

• All lanes : D-3-phosphoglycerate dehydrogenase antibody at 2ug/ml Lane 1 : EC109whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 59 kDa Observed band size : 59 kDa
• Immunohistochemical analysis of paraffin-embedded humankidney using #42388 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting