• Product NamePolycomb complex protein BMI-1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Polycomb complex protein BMI-1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Polycomb complex protein BMI-1 protein
• Target NamePolycomb complex protein BMI-1
• Alternative Names    Polycomb group RING finger protein 4, RING finger protein 51
• Accession No. Swiss-Prot#: P35226
• UniprotP35226
• Gene ID100532731;648;
• Calculated MW 36kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

[1]Characterization and chromosomal localization of the human proto-oncogene BMI-1.Alkema M.J., Wiegand J., Raap A.K., Berns A., van Lohuizen M.Hum. Mol. Genet. 2:1597-1603(1993) [2]Complete sequencing and characterization of 21,243 full-length human c

• All lanes : Polycomb complex protein BMI-1 antibody at 2ug/mlLane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 36 kDa Observed band size: 70 kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting