• Product NameNegative elongation factor B Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Negative elongation factor B polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Negative elongation factor B protein
• Target NameNegative elongation factor B
• Alternative Names    Cofactor of BRCA1
• Accession No. Swiss-Prot#: Q8WX92
• UniprotQ8WX92
• Gene ID25920;
• Calculated MW 64kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

[1] "BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations."Ye Q., Hu Y.-F., Zhong H., Nye A.C., Belmont A.S., Li R.J. Cell Biol. 155:911-921(2001) [2] "Complete sequencing and charact

• All lanes : Negative elongation factor B antibody at 2ug/ml Lane 1 : EC109whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 64 kDa Observed band size : 64 kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting