• Product NameCaspase-3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total Caspase-3 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Caspase-3 protein
• Target NameCaspase-3
• Alternative Names    Apopain, Cysteine protease CPP32Protein Yama, SREBP cleavage activity 1
• Accession No. Swiss-Prot#: P42574
• UniprotP42574
• Gene ID836;
• Calculated MW 30kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Involved in the activation cascade of caspases responsible for apoptosis execution.

[1] CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme.Fernandes-Alnemri T., Litwack G., Alnemri E.S.J. Biol. Chem. 269:30761-30764(1994) [2] Yama/CPP

• All lanes: Caspase-3 antibody at 2ug/ml+mouse brain tissue secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :30kDa observed band size :30kDa
• Immunohistochemical analysis of paraffin-embedded human colon cancer using #42437 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting