• Product NameInterstitial collagenase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Interstitial collagenase polyclonal antibody.
• Target NameInterstitial collagenase
• Alternative Names    Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1
• Accession No. Swiss-Prot#: P03956
• UniprotP03956
• Gene ID4312;
• Calculated MW 51kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

[1] "Cloning and characterization of human tumor cell interstitial collagenase."Templeton N.S., Brown P.D., Levy A.T., Margulies I.M.K., Liotta L.A., Stetler-Stevenson W.G.Cancer Res. 50:5431-5437(1990) [PubMed: 2167156] [Abstract]Cited for: NUCLEOTIDE SE

• All lanes : Interstitial collagenase antibody at 2ug/ml+293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size : 51 kDa Observed band size: 51 kDa
• Immunohistochemical analysis of paraffin-embeded human spleen using #42473 at dilution of 1:100.
• Immunohistochemical analysis of paraffin-embeded human placenta using #42473 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting