• Product NameProtransforming growth factor alpha Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Protransforming growth factor alpha polyclonal antibody.
• Target NameProtransforming growth factor alpha
• Alternative Names    EGF-like TGF, ETGF, TGF type 1
• Accession No. Swiss-Prot#: P01135
• UniprotP01135
• Gene ID7039;
• Calculated MW 18kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

[1] "Human transforming growth factor-alpha: precursor structure and expression in E. coli."Derynck R., Roberts A.B., Winkler M.E., Chen E.Y., Goeddel D.V.Cell 38:287-297(1984) [PubMed: 6088071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1

• All lanes : Protransforming growth factor alpha antibody at 2ug/ml Lane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 18 kDa Observed band size: 30 kDa Additional bands at : 35 kDa.We are unsure as to the identity of this extra band.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting