• Product NameMetalloproteinase inhibitor 2 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Metalloproteinase inhibitor 2 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Metalloproteinase inhibitor 2 protein
• Target NameMetalloproteinase inhibitor 2
• Alternative Names    TIMP2
• Accession No. Swiss-Prot#: P16035
• UniprotP16035
• Gene ID7077;
• Calculated MW 24kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

[1] "Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues."Stetler-Stevenson W.G., Brown P.D., Onisto M., Levy A.T., Liotta L.A.J. Biol. Chem. 265:13933-13938(1990) [2] "cDNA cloning and expressi

• All lanes : Metalloproteinase inhibitor 2 antibody at at 2ug/mlLane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 24kDa Observed band size: 60kDa Additional bands at : 118 kDa. We are unsure as to the identity of this extra band.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting