• Product NameTumor necrosis factor Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Tumor necrosis factor polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Human Tumor necrosis factor protein
• Target NameTumor necrosis factor
• Alternative Names    Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 TNF TNFA, TNFSF2
• Accession No. Swiss-Prot#: P01375
• UniprotP01375
• Gene ID7124;
• Calculated MW 26kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

[1]Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome.Nedospasov S.A., Shakhov A.N., Turetskaya R.L., Mett V.A., Azizov M.M., Georgiev G.P., Korobko V.G., Dobrynin V.N., Filippov S.A., B

• All lanes : Tumor necrosis factor antibody at 2ug/ml+Hela whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size:26kDa Observed band size:26kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting