• Product NameActin, cytoplasmic 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityMs
• SpecificityThe antibody detects endogenous level of total Actin, cytoplasmic 1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant mouse Actin, cytoplasmic 1 protein
• Target NameActin, cytoplasmic 1
• Alternative Names    Actb, Beta-actin
• Accession No. Swiss-Prot#: P60710
• UniprotP60710
• Gene ID11461;
• Calculated MW 42kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

[1] "Nucleotide sequence of a full-length cDNA for mouse cytoskeletal beta-actin mRNA."TokunagaK., Taniguchi H., Yoda K., Shimizu M., Sakiyama S.Nucleic Acids Res. 14:2829-2829(1986) [PubMed: 3754329] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [2

• All lanes : Actin, cytoplasmic 1 antibody at 2ug/ml Lane 1: mouse spleen tissue Lane 2:293T whole cell lysate Lane 3:Hela whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size:42kDa Observed band size:42kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting