• Product NameFKHR(Phospho-Ser319) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FKHR only when phosphorylated at serine 319.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from Human FKHR/FOXO1A.
• Target NameFKHR
• ModificationPhospho
• Alternative Names    FOXO1
• Accession No. Swiss-Prot: Q12778
  NCBI Protein: NP_002006.2
• UniprotQ12778
• Gene ID2308;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 78-82 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

Rena G, et al. (2002) EMBO J 21(9): 2263-2271.
Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607.
Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.

• Western blot analysis of extracts from 3T3 cells untreated or treated with serum starvation using FKHR(Phospho-Ser319) Antibody #11136.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR(Phospho-Ser319) Antibody #11136(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using FKHR(Phospho-Ser319) Antibody #11136.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting