• Product NamePOU domain, class 5, transcription factor 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total POU domain, class 5, transcription factor 1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human POU domain, class 5, transcription factor 1 protein
• Target NamePOU domain, class 5, transcription factor 1
• Alternative Names    Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3
• Accession No. Swiss-Prot#: Q01860
• UniprotQ01860
• Gene ID5460;
• Calculated MW 39kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

[1]"Phosphorylation regulates human OCT4."Brumbaugh J., Hou Z., Russell J.D., Howden S.E., Yu P., Ledvina A.R., Coon J.J., Thomson J.A.Proc. Natl. Acad. Sci. U.S.A. 109:7162-7168(2012). [2]"Serine 111 phosphorylation regulates OCT4A pro

• All lanes: POU domain, class 5, transcription factor 1 antibody at 2ug/ml Lane 1: jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size:39kDa Observed band size:39kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting