• Product NameCadherin-12 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Cadherin-12 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Cadherin-12 protein
• Target NameCadherin-12
• Alternative Names    Brain cadherin, BR-cadherin, Neural type cadherin 2, N-cadherin 2, CDH12
• Accession No. Swiss-Prot#: P55289
• UniprotP55289
• Gene ID1010;
• Calculated MW 87kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

[1] "Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin."Tanihara H., Sano K., Heimark R.L., St John T., Suzuki S.Cell Adhes. Co

• All lanes : Cadherin-12 antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size : 87kDa Observed band size: 87kDa
• Immunohistochemical analysis of paraffin-embeded human kidney using #42564 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting