• Product NameureB Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHelicobacter pylori
• SpecificityThe antibody detects endogenous level of total ureB polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Helicobacter pylori Urease subunit beta protein
• Target NameureB
• Alternative Names    Urea amidohydrolase subunit beta, ureB, hpuB
• Accession No. Swiss-Prot#: P69996
• UniprotP69996
• Gene ID899104;
• Calculated MW 62kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.

[1] "Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity."Labigne A., Cussac V., Courcoux P.J. Bacteriol. 173:1920-1931(1991) [2] "Nucleotide sequence of two genes from Helicobacter pylori

• All lanes : Urease subunit antibody at 2ug/ml+293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size :62kDa Observed band size:62kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting