• Product NameMembrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Target NameMembrane-associated tyrosine-and threonine-specific cdc2-inh
• Alternative Names    Myt1 kinase, PKMYT1, MYT1
• Accession No. Swiss-Prot#: Q99640
• UniprotQ99640
• Gene ID9088;
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:20 - 1:200

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

[1]Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity.Booher R.N., Holman P.S., Fattaey A.J. Biol. Chem. 272:22300-22306(1997) [2]The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes t

• Immunohistochemical analysis of paraffin-embedded human placenta tissue using #42601 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting