• Product NameAFX(Phospho-Ser197) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of AFX only when phosphorylated at serine 197.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.
• Target NameAFX
• ModificationPhospho
• Alternative Names    AFX; FOXO4; AFX1; Afxh;
• Accession No. Swiss-Prot: P98177
  NCBI Protein: NP_001164402.1
• UniprotP98177
• Gene ID4303;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 65kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

• Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody #11137.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody #11137(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody #11137.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting