• Product NameglpK Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityE.coli
• SpecificityThe antibody detects endogenous level of total glpK polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Escherichia coli Glycerol kinase protein
• Target NameglpK
• Alternative Names    ATP:glycerol 3-phosphotransferase, Glycerokinase, glpK
• Accession No. Swiss-Prot#: P0A6F3
• UniprotP0A6F3
• Gene ID948423;
• Calculated MW 62kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Key enzyme in the regulation of glycerol uptake and metabolism.

[1] "Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme."Pettigrew D.W., Ma D.-P., Conrad C.A., Johnson J.R.J. Biol. Chem. 263:135-139(1988) [2] "Structure and regulation of the glpFK operon

• All lanes: Glycerol kinase antibody at 2ug/ml+DH5a whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :62kDa observed band size :62kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting