• Product NameH-2 class I histocompatibility antigen, D-D alpha chain Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total H-2 class I histocompatibility antigen, D-D alpha chain polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Mouse H-2 class I histocompatibility antigen, D-D alpha chain protein
• Target NameH-2 class I histocompatibility antigen, D-D alpha chain
• Alternative Names    H-2D(D), H2-D1
• Accession No. Swiss-Prot#: P01900
• UniprotP01900
• Calculated MW 41kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Involved in the presentation of foreign antigens to the immune system.

[1]"The crystal structure of H-2Dd MHC class I complexed with the HIV-1-derived peptide P18-I10 at 2.4-A resolution: implications for T cell and NK cell recognition."Achour A., Persson K., Harris R.A., Sundbaeck J., Sentman C.L., Lindqvist Y., Schneider G., Kaerre K.Immunity 9:199-208(1998). [2]"The phagosomal proteome in interferon-gamma-activated macrophages."Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.Immunity 30:143-154(2009). [3]"Amino acid sequence of cyanogen bromide fragment CN-C (residues 24-98) of the mouse histocompatibility antigen H-2Dd. A comparison of the amino-terminal 100 residues of H-2Dd, Dd, Kd, and Kb reveals discrete areas of diversity."Nairn R., Nathenson S.G., Coligan J.E.Biochemistry 20:4739-4745(1981).

• All lanes: H-2 class I histocompatibility antigen, D-D alpha chain antibody at 2ug/ml+mouse spleen tissue secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :41kDa observed band size :41kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting