• Product NameSPARC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SPARC protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human SPARC
• Target NameSPARC
• Alternative Names    ON
• Accession No. Swiss-Prot#: P09486
  Gene ID: 6678
• UniprotP09486
• Gene ID6678;
• Calculated MW 35kd
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.?

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: A375 cells
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #42713 at dilution 1/25.
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #42713 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting