• Product NameANKRD1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ANKRD1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human ANKRD1
• Target NameANKRD1
• Alternative Names    ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
• Accession No. Swiss-Prot#: Q15327
  Gene ID: 27063
• UniprotQ15327
• Gene ID27063;
• Calculated MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:200

The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system.?

• Gel: 8%SDS-PAGE
  Lysate: 60 μg
  Lane 1-3: Mouse lung tissue, NIH/3T3 cells, hepg2 cells
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 3 seconds
• Immunohistochemical analysis of paraffin-embedded Human Esophagus cancer tissue using #42714 at dilution 1/100.
• Immunohistochemical analysis of paraffin-embedded Human Gastric cancer tissue using #42714 at dilution 1/100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting